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gene therapy question

Dr. J.P. Clewley jclewley at crc.ac.uk
Tue May 24 18:19:46 EST 1994


In article <2rrmho$pcd at news.u.washington.edu>, frv at u.washington.edu (Franklin Vincenzi) writes:
> Dr. J.P. Clewley <jclewley at crc.ac.uk> wrote:
> 
> Some helpful advice, and then
> 
> >What does anyone else think?
> 
> I think I'm glad I asked what the lot of you think about this!  Thanks 
> for the crash course.
> 
> Regards, Franklin


One thing I forgot to mention is that its more difficult to engineer
a negative strand virus than a positive strand one. If its +ve, you
can make a cDNA clone & transfect it in cells & recover progeny virus.
Works for alphaviruses (eg. Sindbis) picornaviruses (e.g. polio),
retroviruses (e.g. HIV). Recovering -ve strand virus from transfected
DNA has not been done reproducibly, as far as I know. Is there a
confirmed example I'm missing?

Cheers, Jon




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