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Green fluorescent protein

Mark S. Bilk msb at netcom.com
Sun May 29 20:42:08 EST 1994


In article <94141.220432U32472 at uicvm.uic.edu>,
Alan Hinds  <U32472 at uicvm.uic.edu> wrote:
>I am posting this for a friend:
>
>I am interested in putting the green fluorescent protein from the
>bioluminescent jellyfish Aequorea victoria into adenovirus for
>infection of mammalian cells.  

Pardon a somewhat naive question, but isn't adenovirus one of the 
viruses that causes "the common cold" or upper respiratory flu?
Isn't it thus extremely contagious among humans?

If so, isn't it extremely dangerous to add a whole new gene to this 
virus unless one is absolutely certain that this protein could not 
have any adverse effects in people, should the virus escape into 
the human population?

Is genetic modification of adenovirus a routine procedure these
days for the purpose of exerting effects on mammalian cells?

>I have heard that the stability of this
>protein in mammalian cells has been somewhat of a problem.  Has
>anyone had any experience with this and/or constructed more
>stable expression vectors for this protein in mammalian cells?
>Thanks in advance for any responses.





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