titer of baculoviruses

Jay Evans evansj at bcc.orst.edu
Wed Apr 12 17:46:10 EST 1995


In article <yeagerm-1004951656040001 at stubbs1.tjh.tju.edu>,
yeagerm at jeflin.tju.edu (Mark Yeager) wrote:

> In article <3lubeu$l7h at mail.boku.ac.at>, steink at mail.boku.ac.at (Herta
> STEINKELLNER) wrote:
> 
> > In my baculovirus working stock I found a titer of more than 10 to the tenth 
> > (10 000 000 000) pfu / ml. Does anybody know if it is possible to habe such
> > a high titer? 
> > The highest titer I found in literature is 5 times ten
> > to the eighth (5x100 000 000).
> 
> The highest titers I have seen in practice or published is about 2x10^8. I
> suspect that you may have made a dilution error or contaminated your
> dilutions somehow.
> 
> --  -.--
> Mark Yeager ->   yeagerm at jeflin.tju.edu
> Pathology and Cell Biology, Thomas Jefferson Univ., Phila., PA

Using AcMNPV grown in SF-9 cells (TC100 medium) our lab usually gets titers
around 1->3 X10^9 pfu/ml.  The highest I have seen is 3.4X10^9 pfu/ml.
-- 
Jay Evans
evansj at bcc.orst.edu



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