In <ECS9509070913A at utoronto.ca> sophia.mittas at utoronto.ca (James
Campbell) writes:
>>>I am trying to get clear micrographs of the fine fiber proteins which
extend from adenoviruses.
>>I am currently using carbon-coated grids and a Philips300 EM and have
found that staining the virus with
>sodium silicotungstate produces very nice definition of the virus
capsid, however, the fibres are seldom
>visible. Also, the few times I do see the fibres, they don't show up
on the micrographs, or when they do
>they are very blurry. I have noticed that when taking pictures, the
carbon film tends to drift.
>>If you have any suggestions on how to prevent this drift or know of
any other techniques for adenovirus
>fiber staining, I would be very grateful.
>>Sophia Mittas
>sophia.mittas at utoronto.ca>>>Sophia
Try gold (Au).
Charlie
Charles P. McCarthy
Healthcare Consulting and
Medical Research
Carmichael, CA, USA
