To anyone who would like to amplify and clone the entire HIV-1 in a single step:
Due to widespread interest in the topic, we are placing a preprint of our
manuscript (To appear in Virology, October 20th (c) 1995, Academic Press,
inc.) named:
" Recovery of Virtually Full Length HIV-1 Provirus of Diverse Subtypes
from Primary Virus Cultures Using the Polymerase Chain Reaction "
By
Mika O. Salminen 1, Christine Koch 1, Eric Sanders-Buell 1, Philip K.
Ehrenberg 1, Nelson L. Michael 2, Jean K. Carr 1, Donald S. Burke 2 and
Francine E. McCutchan 1.
at
1. Henry M. Jackson Foundation for the Advancement of Military Medicine
and 2.Division of Retrovirology, Walter Reed Army Institute of Research,
Rockville,
MD. 20850
on our new WWW webpage. It includes all graphics and text of the
manuscript as well as detailed Lab-protocols for the actual amplification
and cloning of the products.
The webpage adress is: http://hivgenome.hjf.org/
and it includes other topics which might be of interest for virologists
Please have a look!
Mika Salminen, Ph.D.
Research Fellow
Henry M. Jackson Foundation and the MMCARR
Retrovirology, Global Molecular Epidemiology
1600 East Gude Drive, 20850 Rockville, MD
Phone: 301-217-9410, ext. 1045
Fax: 301-762-7460