help with Hirt protocol?

Leandros Arvanitakis larvanit at mail.med.cornell.edu
Mon Sep 25 11:32:10 EST 1995

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would anyone out there have an optimized Hirt protocol for herpesvirus
isolation from B lymphocytes in cell culture? more specifically, I need
help with regard to the optimal duration of NaCl extraction at 4C for a
virus of around 270 kb...

thanks in advance,

Leandros Arvanitakis, Ph.D.
Dept. of Pathology
Cornell Medical Center

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