From dbowers from unf.edu Wed May 7 11:26:10 2008 From: dbowers from unf.edu (Bowers, Doria) Date: Wed May 7 11:31:20 2008 Subject: [Virology] particle to pfu ratios Message-ID: <783CE756031AB141B53257D89039DD75075EFFA3@DIAMOND.unfcsd.unf.edu> We are wondering what the particle:PFU ratio is for porcine parvovirus. Any ideas how to acquire this statistic? Doria Bowers From M.Dunowska from massey.ac.nz Fri May 9 17:49:55 2008 From: M.Dunowska from massey.ac.nz (Dunowska, Magda) Date: Fri May 9 23:08:00 2008 Subject: [Virology] RE: Virology Digest, Vol 29, Issue 1 In-Reply-To: <200805071704.m47H4AQ24578@net.bio.net> References: <200805071704.m47H4AQ24578@net.bio.net> Message-ID: <92FDFD8B26EB6542B1E1BF017BB998D1480CA23C93@TUR-EXCHMBX.massey.ac.nz> Doria, If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought... Magda -----Original Message----- From: virology-bounces@oat.bio.indiana.edu [mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of virology-request@oat.bio.indiana.edu Sent: Thursday, 8 May 2008 5:04 a.m. To: virology@magpie.bio.indiana.edu Subject: Virology Digest, Vol 29, Issue 1 Send Virology mailing list submissions to virology@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/virology or, via email, send a message with subject or body 'help' to virology-request@net.bio.net You can reach the person managing the list at virology-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Virology digest..." Today's Topics: 1. particle to pfu ratios (Bowers, Doria) ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 May 2008 12:26:10 -0400 From: "Bowers, Doria" Subject: [Virology] particle to pfu ratios To: Message-ID: <783CE756031AB141B53257D89039DD75075EFFA3@DIAMOND.unfcsd.unf.edu> Content-Type: text/plain; charset="US-ASCII" We are wondering what the particle:PFU ratio is for porcine parvovirus. Any ideas how to acquire this statistic? Doria Bowers ------------------------------ _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology End of Virology Digest, Vol 29, Issue 1 *************************************** From M.Dunowska from massey.ac.nz Fri May 9 18:14:16 2008 From: M.Dunowska from massey.ac.nz (Dunowska, Magda) Date: Fri May 9 23:08:05 2008 Subject: [Virology] RE: particle to pfu ratio In-Reply-To: <200805071704.m47H4AQ24578@net.bio.net> References: <200805071704.m47H4AQ24578@net.bio.net> Message-ID: <92FDFD8B26EB6542B1E1BF017BB998D1480CA23C94@TUR-EXCHMBX.massey.ac.nz> Doria, If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought... Magda ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 May 2008 12:26:10 -0400 From: "Bowers, Doria" Subject: [Virology] particle to pfu ratios To: Message-ID: <783CE756031AB141B53257D89039DD75075EFFA3@DIAMOND.unfcsd.unf.edu> Content-Type: text/plain; charset="US-ASCII" We are wondering what the particle:PFU ratio is for porcine parvovirus. Any ideas how to acquire this statistic? Doria Bowers ------------------------------ _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology End of Virology Digest, Vol 29, Issue 1 *************************************** From randhawapa from upmc.edu Sat May 10 00:43:16 2008 From: randhawapa from upmc.edu (Randhawa, Parmjeet) Date: Sat May 10 14:02:13 2008 Subject: [Virology] RE: particle to pfu ratio In-Reply-To: <92FDFD8B26EB6542B1E1BF017BB998D1480CA23C94@TUR-EXCHMBX.massey.ac.nz> References: <200805071704.m47H4AQ24578@net.bio.net> <92FDFD8B26EB6542B1E1BF017BB998D1480CA23C94@TUR-EXCHMBX.massey.ac.nz> Message-ID: <58085D8E8D09D34497D5595559B1A38B0E1796C7@1upmc-msx5.acct.upmchs.net> I think parvovirus is smaller than 5.2 kb, more like 2-3 kb. Virus particles could also be quantitated by electron microscopy but that will not distinguish viable from non-viable virions. Parmjeet Randhawa, M.D. Professor of Pathology, Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC-Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213. Phone: 412 647 7646 Fax: 412 647 5237 -----Original Message----- From: virology-bounces@oat.bio.indiana.edu [mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of Dunowska, Magda Sent: Friday, May 09, 2008 7:14 PM To: virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: [Virology] RE: particle to pfu ratio Doria, If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought... Magda ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 May 2008 12:26:10 -0400 From: "Bowers, Doria" Subject: [Virology] particle to pfu ratios To: Message-ID: <783CE756031AB141B53257D89039DD75075EFFA3@DIAMOND.unfcsd.unf.edu> Content-Type: text/plain; charset="US-ASCII" We are wondering what the particle:PFU ratio is for porcine parvovirus. Any ideas how to acquire this statistic? Doria Bowers ------------------------------ _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology End of Virology Digest, Vol 29, Issue 1 *************************************** _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology From M.Dunowska from massey.ac.nz Sun May 11 16:55:49 2008 From: M.Dunowska from massey.ac.nz (Dunowska, Magda) Date: Sun May 11 19:42:01 2008 Subject: [Virology] RE: particle to pfu ratio In-Reply-To: <58085D8E8D09D34497D5595559B1A38B0E1796C7@1upmc-msx5.acct.upmchs.net> References: <200805071704.m47H4AQ24578@net.bio.net> <92FDFD8B26EB6542B1E1BF017BB998D1480CA23C94@TUR-EXCHMBX.massey.ac.nz> <58085D8E8D09D34497D5595559B1A38B0E1796C7@1upmc-msx5.acct.upmchs.net> Message-ID: <92FDFD8B26EB6542B1E1BF017BB998D1480CA23D9B@TUR-EXCHMBX.massey.ac.nz> Hi everybody, With respect to Parmjeet's email below: I got the 5.2 from a description in a veterinary virology book, but after your email I did check that info. So, parvovirus genomes are 5 kbp long according to the latest report on virus taxonomy (http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/00.050.1.01.htm) and the available full genome sequences of porcine parvovirus on GenBank are about this length (a link to a recent submission of a whole sequence of 5075 bp: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=9628826). Also, I am not sure how accurate the EM would be for quantifying viral particles, as it is a pretty insensitive tool and one needs at least 10*6 particles/mL to be even able to see them under EM, but I would be very interested to know whether or not anybody out there have used it for this purpose? Magda Dunowska, LW (vet), PhD Senior Lecturer in Veterinary Infectious Diseases (Virology) Institute of Veterinary, Animal and Biomedical Sciences Te Kura Mâtauranga Kararehe Massey University Palmerston North New Zealand Phone : (06) 356-9099 ext 7571 Website : http://ivabs.massey.ac.nz -----Original Message----- From: Randhawa, Parmjeet [mailto:randhawapa@upmc.edu] Sent: Saturday, 10 May 2008 5:43 p.m. To: Dunowska, Magda; virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: RE: [Virology] RE: particle to pfu ratio I think parvovirus is smaller than 5.2 kb, more like 2-3 kb. Virus particles could also be quantitated by electron microscopy but that will not distinguish viable from non-viable virions. Parmjeet Randhawa, M.D. Professor of Pathology, Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC-Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213. Phone: 412 647 7646 Fax: 412 647 5237 -----Original Message----- From: virology-bounces@oat.bio.indiana.edu [mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of Dunowska, Magda Sent: Friday, May 09, 2008 7:14 PM To: virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: [Virology] RE: particle to pfu ratio Doria, If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought... Magda ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 May 2008 12:26:10 -0400 From: "Bowers, Doria" Subject: [Virology] particle to pfu ratios To: Message-ID: <783CE756031AB141B53257D89039DD75075EFFA3@DIAMOND.unfcsd.unf.edu> Content-Type: text/plain; charset="US-ASCII" We are wondering what the particle:PFU ratio is for porcine parvovirus. Any ideas how to acquire this statistic? Doria Bowers ------------------------------ _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology End of Virology Digest, Vol 29, Issue 1 *************************************** _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology From Protoman2050 from gmail.com Sat May 10 17:08:56 2008 From: Protoman2050 from gmail.com (douglas) Date: Mon May 12 10:58:48 2008 Subject: [Virology] Could this be a possible treatment for HIV/AIDS? Message-ID: <0cc443e2-f516-42cd-959e-afb4e7571632@x19g2000prg.googlegroups.com> Could this be a possible treatment for HIV/AIDS: Synthesize a peptide nucleic acid antisense oligonucleotide for HIV’s gag gene’s mRNA. The reason for using PNA is that it is substantially more resistant to enzyme degradation by nucleases and proteases. The reason for choosing the gag gene is that it’s proteins, p24, p17, p7, and p6, code for the basic physical infrastructure of HIV; w/o these key proteins, there is no HIV. Then, encapsulate the oligonucleotides in liposomes studded w/ anti-CD4 antibodies. This will ensure 1) toxicity is limited—cf Ambisome, the liposomal preperation of amphotericin B—2) the biologic goes only where it’s needed, which is the cytoplasm of CD4+ T-cells. Decorating the liposome w/ anti-CD4 antibodies will trigger endocytosis, in my limited knowledge, at least. I predict low toxicity and high efficacy in reducing the patient’s viral load to hopefully 0 copies/ml. Maybe, when I'm doing my PhD or MD dissertation --a long ways off, see below--, I could do it on this, patent the resulting product --ie the PNA oligonucleotide itself--, and license it to Genentech or Hybridon for further development into a marketable biologic. What are your thoughts? Should LNA instead of PNA be used? Cell- penetrating peptides vs. liposomes? Targeting the gag gene vs. targeting the pol gene? Using anti-CD4 mAbs vs. anti-gp160 mTcRs as a drug targeting device? Are there any publications about this subject? Another drug could be a mTcR for gp160, linked to FasL...this should cause formation of DISC, leading to the cell's eventual apoptosis. And since it's linked to an mTcR that only recognizes gp160, only HIV- infected cells will undergo apoptosis. And since apoptosis is an organized "format c:" of the cell, the virons will also be destroyed!!! In your expert opinions, do you believe these will cause remission of HIV/AIDS? Partial or complete? And, just for fun, what do you think the generic names would be for these biologics? Brand names? BTW, if this helps you answer, I'm a 16.5 year old community college freshman who hopes to have a career as a physician-scientist. Thanks!!!! From randhawapa from upmc.edu Sun May 11 21:27:17 2008 From: randhawapa from upmc.edu (Randhawa, Parmjeet) Date: Mon May 12 10:58:53 2008 Subject: [Virology] RE: particle to pfu ratio In-Reply-To: <92FDFD8B26EB6542B1E1BF017BB998D1480CA23D9B@TUR-EXCHMBX.massey.ac.nz> References: <200805071704.m47H4AQ24578@net.bio.net><92FDFD8B26EB6542B1E1BF017BB998D1480CA23C94@TUR-EXCHMBX.massey.ac.nz><58085D8E8D09D34497D5595559B1A38B0E1796C7@1upmc-msx5.acct.upmchs.net> <92FDFD8B26EB6542B1E1BF017BB998D1480CA23D9B@TUR-EXCHMBX.massey.ac.nz> Message-ID: <58085D8E8D09D34497D5595559B1A38B0E1796C8@1upmc-msx5.acct.upmchs.net> Magda, Thanks for the correction. I was confusing genome size with virion diameter. Parvovirus is much smaller (approximately 20 nm versus 50 nm). The low sensitivity of electron microscopy is an issue for clinical diagnosis but should not be for virus grown in culture. I do not have personal experience in quantitation or the accuracy thereof. I will be interested in data on the accuracy of PFU measurements too. Since it involves serial dilution of a stock the accuracy would depend on how many dilution steps are used before actual plating. Parmjeet Parmjeet Randhawa, M.D. Professor of Pathology, Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC-Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213. Phone: 412 647 7646 Fax: 412 647 5237 -----Original Message----- From: virology-bounces@oat.bio.indiana.edu [mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of Dunowska, Magda Sent: Sunday, May 11, 2008 5:56 PM To: Randhawa, Parmjeet; virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: RE: [Virology] RE: particle to pfu ratio Hi everybody, With respect to Parmjeet's email below: I got the 5.2 from a description in a veterinary virology book, but after your email I did check that info. So, parvovirus genomes are 5 kbp long according to the latest report on virus taxonomy (http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/00.050.1.01.htm) and the available full genome sequences of porcine parvovirus on GenBank are about this length (a link to a recent submission of a whole sequence of 5075 bp: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=9628826). Also, I am not sure how accurate the EM would be for quantifying viral particles, as it is a pretty insensitive tool and one needs at least 10*6 particles/mL to be even able to see them under EM, but I would be very interested to know whether or not anybody out there have used it for this purpose? Magda Dunowska, LW (vet), PhD Senior Lecturer in Veterinary Infectious Diseases (Virology) Institute of Veterinary, Animal and Biomedical Sciences Te Kura Mâtauranga Kararehe Massey University Palmerston North New Zealand Phone : (06) 356-9099 ext 7571 Website : http://ivabs.massey.ac.nz -----Original Message----- From: Randhawa, Parmjeet [mailto:randhawapa@upmc.edu] Sent: Saturday, 10 May 2008 5:43 p.m. To: Dunowska, Magda; virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: RE: [Virology] RE: particle to pfu ratio I think parvovirus is smaller than 5.2 kb, more like 2-3 kb. Virus particles could also be quantitated by electron microscopy but that will not distinguish viable from non-viable virions. Parmjeet Randhawa, M.D. Professor of Pathology, Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC-Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213. Phone: 412 647 7646 Fax: 412 647 5237 -----Original Message----- From: virology-bounces@oat.bio.indiana.edu [mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of Dunowska, Magda Sent: Friday, May 09, 2008 7:14 PM To: virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: [Virology] RE: particle to pfu ratio Doria, If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought... Magda ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 May 2008 12:26:10 -0400 From: "Bowers, Doria" Subject: [Virology] particle to pfu ratios To: Message-ID: <783CE756031AB141B53257D89039DD75075EFFA3@DIAMOND.unfcsd.unf.edu> Content-Type: text/plain; charset="US-ASCII" We are wondering what the particle:PFU ratio is for porcine parvovirus. Any ideas how to acquire this statistic? Doria Bowers ------------------------------ _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology End of Virology Digest, Vol 29, Issue 1 *************************************** _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology From randhawapa from upmc.edu Sun May 11 21:27:17 2008 From: randhawapa from upmc.edu (Randhawa, Parmjeet) Date: Mon May 12 10:58:56 2008 Subject: [Virology] RE: particle to pfu ratio In-Reply-To: <92FDFD8B26EB6542B1E1BF017BB998D1480CA23D9B@TUR-EXCHMBX.massey.ac.nz> References: <200805071704.m47H4AQ24578@net.bio.net><92FDFD8B26EB6542B1E1BF017BB998D1480CA23C94@TUR-EXCHMBX.massey.ac.nz><58085D8E8D09D34497D5595559B1A38B0E1796C7@1upmc-msx5.acct.upmchs.net> <92FDFD8B26EB6542B1E1BF017BB998D1480CA23D9B@TUR-EXCHMBX.massey.ac.nz> Message-ID: <58085D8E8D09D34497D5595559B1A38B0E1796C8@1upmc-msx5.acct.upmchs.net> Magda, Thanks for the correction. I was confusing genome size with virion diameter. Parvovirus is much smaller (approximately 20 nm versus 50 nm). The low sensitivity of electron microscopy is an issue for clinical diagnosis but should not be for virus grown in culture. I do not have personal experience in quantitation or the accuracy thereof. I will be interested in data on the accuracy of PFU measurements too. Since it involves serial dilution of a stock the accuracy would depend on how many dilution steps are used before actual plating. Parmjeet Parmjeet Randhawa, M.D. Professor of Pathology, Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC-Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213. Phone: 412 647 7646 Fax: 412 647 5237 -----Original Message----- From: virology-bounces@oat.bio.indiana.edu [mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of Dunowska, Magda Sent: Sunday, May 11, 2008 5:56 PM To: Randhawa, Parmjeet; virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: RE: [Virology] RE: particle to pfu ratio Hi everybody, With respect to Parmjeet's email below: I got the 5.2 from a description in a veterinary virology book, but after your email I did check that info. So, parvovirus genomes are 5 kbp long according to the latest report on virus taxonomy (http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/00.050.1.01.htm) and the available full genome sequences of porcine parvovirus on GenBank are about this length (a link to a recent submission of a whole sequence of 5075 bp: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=9628826). Also, I am not sure how accurate the EM would be for quantifying viral particles, as it is a pretty insensitive tool and one needs at least 10*6 particles/mL to be even able to see them under EM, but I would be very interested to know whether or not anybody out there have used it for this purpose? Magda Dunowska, LW (vet), PhD Senior Lecturer in Veterinary Infectious Diseases (Virology) Institute of Veterinary, Animal and Biomedical Sciences Te Kura Mâtauranga Kararehe Massey University Palmerston North New Zealand Phone : (06) 356-9099 ext 7571 Website : http://ivabs.massey.ac.nz -----Original Message----- From: Randhawa, Parmjeet [mailto:randhawapa@upmc.edu] Sent: Saturday, 10 May 2008 5:43 p.m. To: Dunowska, Magda; virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: RE: [Virology] RE: particle to pfu ratio I think parvovirus is smaller than 5.2 kb, more like 2-3 kb. Virus particles could also be quantitated by electron microscopy but that will not distinguish viable from non-viable virions. Parmjeet Randhawa, M.D. Professor of Pathology, Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC-Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213. Phone: 412 647 7646 Fax: 412 647 5237 -----Original Message----- From: virology-bounces@oat.bio.indiana.edu [mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of Dunowska, Magda Sent: Friday, May 09, 2008 7:14 PM To: virology@oat.bio.indiana.edu; virology@magpie.bio.indiana.edu Subject: [Virology] RE: particle to pfu ratio Doria, If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought... Magda ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 May 2008 12:26:10 -0400 From: "Bowers, Doria" Subject: [Virology] particle to pfu ratios To: Message-ID: <783CE756031AB141B53257D89039DD75075EFFA3@DIAMOND.unfcsd.unf.edu> Content-Type: text/plain; charset="US-ASCII" We are wondering what the particle:PFU ratio is for porcine parvovirus. Any ideas how to acquire this statistic? Doria Bowers ------------------------------ _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology End of Virology Digest, Vol 29, Issue 1 *************************************** _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology From ziv from Biondvax.com Wed May 28 07:14:12 2008 From: ziv from Biondvax.com (Sagit Ziv-Sefer) Date: Thu May 29 16:22:26 2008 Subject: [Virology] Molecular weight of influenza virus proteins Message-ID: Does some one know the molecular weight of Influenza virus proteins (HA, NP, M, NS etc.) and how do they shown on an SDS-PAGE gel? Thanks Sagit Ziv@biondvax.com From bbx107 from excite.XXXX.com Sat May 31 22:43:49 2008 From: bbx107 from excite.XXXX.com (bob) Date: Sun Jun 1 14:07:44 2008 Subject: [Virology] Re: Molecular weight of influenza virus proteins References: Message-ID: On Wed, 28 May 2008 15:14:12 +0300, "Sagit Ziv-Sefer" wrote: >Does some one know the molecular weight of Influenza virus proteins (HA, >NP, M, NS etc.) and how do they shown on an SDS-PAGE gel? http://www.pnas.org/cgi/content/abstract/75/8/3625 The paper should be freely available there. bob