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Protein Purification : HPLC or FPLC

Susan Chacko susanc at helix.nih.gov
Wed Mar 30 12:06:06 EST 1994

We are considering buying a new HPLC system for use in
protein purification, and are getting conflicting information
about its use for crystallographic protein preps. 

Some crystallographers seem to think that the HPLC columns
are rougher on the proteins, that there is a possibility of
losing the protein, that the columns clog eventually and have
to be replaced at high cost. 
Some biochemists (and the HPLC salespeople, of course!) say that
HPLC can do everything FPLC can, with higher resolution and in
shorter time.

I'd welcome any thoughts and experiences -- do you prefer to use
HPLC or FPLC, and for what reason? Have you had any problems with
your current system? What scale (gms? mgs?) of preps have you
done with the HPLC? What sort of columns do you use?

susanc at helix.nih.gov (note the 'c' after susan)
Bldg 2, Rm 316, NIDDK, NIH, Bethesda, MD 20892
(301) 402-4494 (off)

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