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Protein Purification : HPLC or FPLC

Cornelius Krasel krasel at alf.biochem.mpg.de
Thu Mar 31 08:31:43 EST 1994

Susan Chacko (susanc at helix.nih.gov) wrote:

<about the decision HPLC/FPLC?>

We are using a HPLC by Sykam with FPLC columns by Pharmacia. Works
fine for us. We can do anything (at least that's what I think) we
could do with a FPLC system. If we really need HPLC we just switch
columns :-)

Some people claim that the disadvantage of a HPLC is the contact
between metal and buffer in the pump. I think that this might
be the only advantage of a complete FPLC system. (To be honest,
there is another disadvantage of our system: if you don't be careful
you can easily blow up a FPLC column -- happened to us more
than once).

--Cornelius (not really an expert, but it works :-).

/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de                 fax: +49 89 8578 3795 */
/* "People are DNA's way of making more DNA." (Edward O. Wilson, 1975)    */

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