We have been using light scattering for PROTEIN APPLICATIONS routinely in our
lab. We have not been able to make the measurements of interest to you
(hydrodynamic radius, polydispersity, etc) for OFF-LINE experiments (ie not
employing a gel filtration column) using the Wyatt Dawn instrumentation.
However, we have had GOOD success with off-line protein measurements using the
Protein Solutions Dyna-Pro 801 Instrument (price is ~$20K--which is
substantially cheaper than others). This instrument is quite popular
in many macromolecular crystallography labs. You can contact Protein Solutions
at 804/975-1366. Bill Holmes is the person in our lab who has a great deal of
experience with light scattering applications for proteins....you can contact
him via E mail at WDH17912 at usav01.glaxo.com or 919/941-3927.
amh12478 at usav01.glaxo.com
Glaxo Research Institute