In article <3vb5rl$4c7 at moonbeam.aecom.yu.edu>, Puius <puius> writes:
|> As I up the pH and decrease [salt] I get fewer and bigger stars,
|> but no discrete xtals. Is this a nucleation problem, i.e. should
|> I use compressed air to blow off dust? Any other ideas?
. set up matrices to optimize each crystallization parameter separately,
including but not limited to pH and [salt]. i have even had situations
where [buffer] made a difference. don't forget [protein].
. try different salts.
. try "additives", such as N-octyl glucoside or MPD in small amounts.
. try a larger drop size to slow crystal growth.
. try using your 'stars' for seeding.
. take a trip in the space shuttle.
. make sure your protein is really pure, and centrifuge it just before
setting up crystals to remove any precipitate which could increase
University of California-Irvine
schuller at indigo2.biomol.uci.edu
modern man in a post-modern world