i'm planning to crsytallize and solve the structure of the
protein that i'm working on. there are no homologous structures that
hve been reported for my protein. but i would like to take advantage of
the fact that i have a thioredoxin fusion protein and the structure of
the thioredoxin protein has been determined. my protein is 52.5 kDa and
the thioredoxin is ~11.6 kDa, making the total mass of my fusion protein
at around 64.1 kDa. I would like to know how much homology i need to be
able to reliably use the solved structure of the thioredoxin (comprising
abour 18% of the fusion protein) as molecular replacement to solve the
structure of my protein??? i want to know this in hopes of avoiding
doing heavy atom derivatives (or is that just too inevitable)...please
email to sai at uab.edu . thanks much...
dept. of biochemistry and molecular genetics
univ. of alabama at birmingham...