Cecilia Cabral de Mello (cmello at opal.tufts.edu) wrote:
: Is anyone aware of heavy atom compounds that are particularly suited for
: derivatizing proteins rich in cysteins, e.g. 27 cysteins in a polypeptide
Mercury binds well to free SH, and, I believe to S-S to some ex-
tent. CH3Hg will bind to free SH, but not to S-S and not at divalent
metal binding sites. With that many SH, do you have a metallothionine?
If so, it may bind most divalent metals.
: Also, are there methods that are more gentle than others for situations
: like this?
Adding Hg or CH3Hg is pretty benign, and adding a divalent to a
metallothionine is *very* benign, since those proteins are designed to
sequester those ions. Good luck.