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derivatizing cystein-rich proteins

William Tivol tivol at news.wadsworth.org
Mon May 12 11:42:50 EST 1997

Cecilia Cabral de Mello (cmello at opal.tufts.edu) wrote:
: Is anyone aware of heavy atom compounds that are particularly suited for
: derivatizing proteins rich in cysteins, e.g. 27 cysteins in a polypeptide
: chain?

	Mercury binds well to free SH, and, I believe to S-S to some ex-
tent.  CH3Hg will bind to free SH, but not to S-S and not at divalent
metal binding sites.  With that many SH, do you have a metallothionine?  
If so, it may bind most divalent metals.

: Also, are there methods that are more gentle than others for situations
: like this?

	Adding Hg or CH3Hg is pretty benign, and adding a divalent to a
metallothionine is *very* benign, since those proteins are designed to
sequester those ions.  Good luck.
				Bill Tivol

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