IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Purifying halogenated DNA?

Ng ng at mbi.ucla.edu
Tue Oct 20 17:00:01 EST 1998

     Hi.  I am having some problems purifying bromo and iodo DNA oligomers
(10 and 12 mers).  During purification by anion exchange in 7M urea with
KCl gradient,
I usually see 2-3 significant peaks which overlap.  I don't see these
peaks when purifying non-modified DNA.  What are the components of these
peaks?  And what is a good way to identify the correct peak?  Thanks!

Ho Leung Ng
holeung at ucla.edu

More information about the Xtal-log mailing list

Send comments to us at biosci-help [At] net.bio.net