yeast mt DNA isolation

Christoph Schller CS at abc.univie.ac.at
Fri Jun 18 06:37:48 EST 1993


Dear yeast people
Is there a protocol to isolate mt DNA in large scale without phenol
and without spheroplasting? I want to measure oxidative damage in mt
DNA. I expect 1h on 37  as needed for zymolyase to work will
probably produce artifacts.
Thanks a lot.

Christoph Schueller

PS: Muchas gracias to all those who responded to my earlier question.
-it worked.



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