alpha-factor synchronization

colleen hayase hayase at ewald.mbi.ucla.edu
Sun Aug 14 12:31:07 EST 1994


I'd like to analyze the cell cycle-dependent transcription of certain
genes in S. cerevisiae.  Is it better to use a large dose of alpha-factor
for 2 h or two smaller doses for a total of 3 h?  Any suggestion will be
highly appreciated.



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