breaking yeast

Jeffrey A. Ziehler ziehler at post.its.mcw.edu
Tue Aug 16 11:10:22 EST 1994


Sandy Silverman (sandy at icr.pt.cyanamid.COM) wrote:
: I would give glass beads a try since it is easy.  Just make sure the tubes
: stay cold by vortexing a short time, put on ice, vortex, etc.
: --

In our lab, we break yeast in an osterizer blender with glass beads.
Take frozen cells, mix with 5 cell weights glass beads and one cell volume
buffer, and grind 5x 30sec. each with cooling on a salted ice bath between.
Maintain temperature below 4oC, (temp before each grinding session should
be below 1oC).

good luck.
-- 
jeff z-ler
ziehler at post.its.mcw.edu

waiting for the clu- phenotype to be a target for gene therapy.



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