overcooked plates etc.

Roland J Saldanha rsaldanh at magnus.acs.ohio-state.edu
Wed Aug 24 16:27:57 EST 1994


Difco recommends filter sterilizing the nitrogen base, rather than autoclaving 
it.  I got mushy plates when I autoclaved it together with the agar and now 
only filter sterilize it.  I beleive the notion that the low pH in the media 
hydrolyses the agar may have some merit.  I beleive that yeast grows over a 
fairly wide pH so the low pH of the original formulation may not be a necessity
but a mild selection against other organisms.



More information about the Yeast mailing list