overcooked plates etc.
Roland J Saldanha
rsaldanh at magnus.acs.ohio-state.edu
Wed Aug 24 16:27:57 EST 1994
Difco recommends filter sterilizing the nitrogen base, rather than autoclaving
it. I got mushy plates when I autoclaved it together with the agar and now
only filter sterilize it. I beleive the notion that the low pH in the media
hydrolyses the agar may have some merit. I beleive that yeast grows over a
fairly wide pH so the low pH of the original formulation may not be a necessity
but a mild selection against other organisms.
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