yeast genetics experiments

[Chuck Miller]

Sue Katz (DKatz at Doane.edu) asked:
I am in search of ideas for my molecular genetics course this coming spring.
I want the students to use not just the bacterial strains, but also yeast
mutants.  Is there a source for experiments, or does anyone have some ideas
for experiments and strains?

A good start for introducing students to yeast is:

Methods in Yeast Genetics: A Laboratory Course Manual By Mark D. Rose,
Princeton University; Fred Winston, Harvard Medical School; Philip Hieter,
Johns Hopkins Medical School, from Cold Spring Harbor Press (1990).

CONTENTS
Introduction
Genetic Nomenclature
Experiments
Isolation and Characterization of Auxotrophic, Temperature-sensitive, and
UV-sensitive Mutants; Meiotic Mapping; Mitotic Mapping; Transformation of
Yeast; Complementation and Random Spore Analysis of Histidine Auxotrophs;
Gene Replacement; Nonsense Suppressors of Yeast; The Formation of Zygotes:
Normal and Abnormal Cell Fusion and Karyogamy; Cloning of Yeast Genes by
Complementation; lacZ Gene Fusion Expres-sion in Yeast; Immmunofluorescent
Staining of Yeast Cells; Electrophoretic Karyotyping and Gene Mapping Using
Pulsed-field Gel Electrophoresis
Techniques and Protocols Yeast Transformation; Lithium Acetate Yeast
Transformation; Yeast Colony Hybridization; Yeast DNA Isolations: Yeast DNA
Miniprep (40 ml), Yeast DNA Mini-prep (5 ml), A Ten-minute DNA Prep from
Yeast, Preparation of Yeast Genomic DNA (CsCl Method); Preparation of
Isolated Nuclei from S. cerevisiae; Yeast RNA Isolation; Northern Analysis:
Formaldehyde Agarose Gel, Blotting, and Hybridization to Filters; Alkaline
Southern Blotting Procedure; Scoring Killer Factor; Hydroxyl-amine
Mutagenesis of Plasmid DNA; Assay of b-Galactosidase in Yeast;
Transformation of Bacteria (CaCl2 Method); E. coli Plasmid DNA Miniprep;
Preparation of Chromosome-sized Yeast DNA Molecules in Solid Agarose;
Mapping Cloned DNA Segments by Chromosome "Fragmentation"; Running Gels
using the CHEF Apparatus; Running Gels using the OFAGE Apparatus
Appendices
Media; Stock Preservation; Yeast Genetic Map; Grids; Electrophoretic
Karyotypes of Strains for Southern Blot Mapping; Strains
1990, 198 pp., illus., appendices ISBN 0-87969-354-1 Paper $34 
Return to Book Catalogue  Go to information on ordering books Ingrid
Benirschke / benirsch at cshl.org
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Dr. Charles A. Miller                               
Room 374 J.B. Johnston Bldg. (CBR)
Dept. Environmental Heath Sciences 
Tulane University Medical Center
1501 Canal St. Box SL29
New Orleans, LA. 70112
Ph. 504-585-6942 Fax 504-585-6939
rellim at mailhost.tcs.tulane.edu
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