In article <84417CF4E00018BE at if1.ufrgs.br>, DAVI at IF1.UFRGS.BR writes:
> Dear nets,
>> The leu2-3,-112 double mutation is a standard marker used in S. cerevisiae
> strains.
> I lost track of an article describing which mutations correspond to
> leu2-3,-112.
> Does anybody know this article or any other reference ?
> If I made a mistake thinking this double mutation is already described,
> does anybody know the type and position of these mutations ?
> Thanks.
>> David Keszenman-Pereyra
> Centro de Biotecnologia
> Av Bento Gencalves, 9500,
> Campus do Vale/UFRGS, 91500
> Porto Alegre, RS, Brazil
> Fax 55 (051) 336-2779
> e-mail DAVI at IF1.UFRGS.BR>> Knowledge is power (Bacon)
The position of the leu2-3 mutation is a +1 insertion of a G after
nucleotide 246 of the coding sequence. This is very close to the KpnI
site. All I know about the leu2-112 mutation is that it was also
induced by ICR170 mutagenesis and so is likely to be a +1 frameshift
insertion in a string of G residues. It maps somwehere 3' to the
EcoRI site in the gene. Mike Culbertson at U. Wisconsin may have more
information on this allele.
Hannah Klein
NYU Medical Center
NY, NY
e-mail KLEIN at MCCLB0.MED.NYU.EDU
