counterselection in yeast

Wolfgang Hilt Uni Stuttgart 685-4388 Hilt at PO.UNI-STUTTGART.DE
Thu Apr 6 11:43:56 EST 1995


Hello Netters, we want to do a plasmid shuffling experiment but can not use 
the URA3/5FOA system for counterselection. Has anyone experience with 
counterselection using LYS2/alpha-aminoadipate, CHY2 and cycloheximide 
resistance or CAN1 and canavanine resistance. Can you send your message also 
to my e-mail adress, Thank you wolfgang.

 
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0000    Wolfgang Hilt          phone: 0711/685-4388                       0000
0000    Inst. fŸr Biochemie    fax:   0711/685-4392                       0000
0000    UniversitŠt Stuttgart  e-mail: hilt at po.uni-stuttgart.de           0000
0000    70569-Stuttgart                                                   0000
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