(no subject)

[Enoch Gao]

--  
Ryan O'Neill	 
Depart.of Biochem.,McGill University, Montreal, Canada  
(lab)514-398-7280 Fax:514-398-7384 (H)514-286-1973 
e-mail: oneill at medcor.mcgill.ca 
 
I am interested in finding out whether or not there exists a plasmid prep  
from yeast that eliminates the contaminating genomic DNA that is present 
in the phenol/chloroform/glass beads protocol. I am trying to transform 
e. coli (HB101) with this DNA and it will not work.  I am attempting to  
isolating the plasmid pGAD10 containing an insert which possibly   
interacts with my bait in the two-hybrid system. 
                                Thank you.