Yeast Cells: Template source for PCR.

NAGU at VAX2.CONCORDIA.CA NAGU at VAX2.CONCORDIA.CA
Thu Jan 5 22:26:14 EST 1995


Hello everyone,
	I have been using the Yeast Genomic DNA for PCR.  I find the 
preparation of Yeast Genomic DNA is a bit of time consuming process.  
I am looking for a rapid method where I could pick up the yeast colony
from the plate and lyse the cells (in situ way), then use that cell lysate
as a source of template for PCR.  I heard this sort of method is possible
with E.coli.  I am woundering 
ndering is there any method describes about using the
whole yeast cells directly for PCR?
Thanks in advance for any help.
Nagu
Nagu at vax2.concordia.ca



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