Difference between 2 Strain?

WANGHO at UCBEH.SAN.UC.EDU WANGHO at UCBEH.SAN.UC.EDU
Fri Jan 27 14:20:27 EST 1995


Re:  What is the difference between yeast strain ?

Hi There!

I am a newer in yeast but working on the yeast two hybrid system for almost
three years.  A lot of exciting data gave me a big picture about the cross
talking among the proteins I am interested in.  But one interaction bother me
for a while.  I guess this might be a potential problem or could be a
commo-problem in the future using yeast two hybrid system.

1. I detected the interaction between Protein A and Protein B in Yeast strain,
PCY2.  The b-galactosidase activity induced by this interaction is beatiful.

2. Since some other strains have been developed (esp. using his and gal for
double selection), I tried one.  All positive are positive,and all negative are
negative.  Most interaction in my work showed positive.  But A-B interaction
showed negative (both in his and b-galactosidase).

3. I made a conclusion that there is no direct A-B interaction but a complex
containing A and B is formed, and the A-B complex is medicated by other
factors.

Above is the experiment and conclusion.  It appears to be perfect to tell this
story in my next publication.  But, as I said, I am an user not a "yeastist". 
So I basically want ask myself and "yeastists" three questions.

1. Is the difference between strains enough to giving these difference? I
understand that the difference between two animal cells might be trillion.  Can
some one tell me at what level the yeast strains show difference?

2. Am I so lucky to find this complex but an indirect interaction by using the 
second strain, which I pick randomly "one day"?  

3. Obiviously, one like to know what is the third factor(s) mediating this
interaction.  Any suggestion to provide that there is such factor(s) before I
screening the yeast library (beacuse the screen is not the first choice, at
least for me)? 

Any suggestion or comments are appreactiated.  If this is a true problem in the
using of two hybrid system, I wish this should be paid attention to.  The
false-positive might means more than what we thought.

By the way, I can read "yeast-net" through gopher.  You can send mail to me or
"yeast-net".

Thanks again!

Hong F. Wang
Department of Cell Biology, Neurobiology, and Anatomy
University of CIncinnati College of Medicine
231 Bethesda Ave.
Cincinnati, OH 45267-0521

Phone: (513)558-6787
Fax: (513)558-4454
E-mail: wangho at ucbeh.san.uc.edu




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