How do I select the Leu- cells?
S. L. Rennie
rennie at bldghsc.lan1.umanitoba.ca
Tue Jul 18 18:20:37 EST 1995
In article <9507141129.AA18235 at tansei1.tansei.cc.u-tokyo.ac.jp>,
yohsumi at TANSEI.CC.U-TOKYO.AC.JP (Yohsumi) wrote:
> Dear Yeast people,
>
> I want to know how to select the Leu- cells by plate assay.
> Does anyone know a reagent for counter-selection of Leu- colonies?
> I also want to know about Leu analog, too.
>
> Thanks.
>
> Satoshi Kametaka
--------------------------------
Replicate results on to SC-Leu media...the ones that don't grow would likely be
leu- strains. Pick these from master plate (YPAD?)
Would be interested if there is some "counter selection reagent" though.?
Sharon
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