Anaerobic growth of S. cerevisiae
bkrett at ACNS.ColoState.EDU
Fri Jul 28 12:59:26 EST 1995
> > I am attempting to grow S. cerevisiae anaerobically. Is there
> >any specific media or media supplements that I should use? Any
> >information would be greatly appreciated.
> >Natalie J. Morey nmorey at bimcore.emory.edu
> >Dr. Jinks-Robertson lab
> >Emory University
> >Atlanta, GA
I am currently working on a strain of yeast that has a gene knock
out of the precursor to the CYC1 gene. Aerobic growth can be created by
growing on a nonfermentable carbon source adding the metabolic precursor.
Anerobic growth occurs normally in these cells as long as they
are growht on a fermentable carbon source. If you can use these cells let
me know and I will see about sending them out to you.
bkrett at holly.colostate.edu
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