yeast trna

[Tim Ulmasov]

In article <thys.3.2FDEFC50 at molgen.biologie.uni-marburg.de>,
thys at molgen.biologie.uni-marburg.de (Matthias K„mpfer) wrote:

> Has anybody informations about processing of trna in yeast ?
> I want to construct a suppressor trna and clone it in to a Pol 
> II expressioncasette and in this connection not to take account of the 5' 
> and 3' flanking sequences of the trna. In E.Coli have the flanking sequences 
> no influence on the correct processing of the prae trna.
> Thanks a lot. 

I'd be very interested to know examples where tRNA suppressor gene was
functional under the control of Pol II promoter. If you know any, could you post
them in this newsgroup, or respond directly to bichguil at mizzou1.missouri.edu

Tim Ulmasov
University of Missouri-Columbia