HIS3 upstream sequence

[Karen Sirum-connolly]

Todd DeZwaan (DEZWAAN_T.a1.mscf.upenn.edu) wrote:
: I am constructing a plasmid which carries the HIS3 gene from the vector
: pJJ215.  This is one of a series of constructs made by Jones and Prakash
: and carries the HIS3 gene as a BamHI fragment in pUC18.  I would like to
: piece together the full sequence of my final construct but have been
: unable to find sequence data for the region between the 5'-BamHI site
: (-491) and the HIS3 ATG.  Does anybody know where I can find this
: sequence?  Thanks for your help.

: Todd Dezwaan
 
  Dear Todd,
  See GenBank Accession # L19947.  This is the sequence of the PET56 
  gene which is adjacent to HIS3 in the yeast nuclear genome.

  Karen Sirum-Connolly
  Dept. of Biochemistry and Molecular Biology
  Univ. of Massachusetts
  Amherst, MA 01003