Protein tags in yeast

[Cynthia Bolognese]

We use the FLAG epitope in our protein. It works fine in westerns, but I 
am having one heck of a time immunoprecipitating. Another grad student 
tried to purify the protein using an anti-flag column, but was not able 
to. I wish had a success story to share.Since I don't, I'd like to know 
what success/failure other people have had. It might help me ip my 
tagged protein.