Forwarded Yeast Question from rec.crafts.brewing
rbutler at cableregina.com
Thu Dec 5 02:34:00 EST 1996
The following appeared in rec.crafts.brewing... any experts care to
Aseptic yeast handlers -- a simple question....
I am now in the process of archiving yeast stocks both at -80^C
30% glycerol and in distilled water at room temp. In the latter
am using 50 ml serum vials with autoclaved septa as containers. This
handling of yeast as aseptic as possible from the "water bottles".
My question for those handling their yeast with hypodermic
did John Isenhour in the Zymurgy 'Yeast & Beer' special issue [back
special issues meant something!]) is what GAUGE needle is acceptable
permit passage of undamaged yeast cells, yet be small enough to not
completely destroy the septa on my vials?? The article Isenhour wrote
generally informative, however this detail (in my estimation, an
one) was omitted. Having too small a needle bore will likely damage
cells or clog frequently.
Anyone handling their yeast in this manner care to comment???
Thanks in advance for any information anyone may provide...
[P.S. First time in over a year that I've been nearly caught up on
- It could be worse I guess.....]
Robert S. Wallace
Associate Professor of Botany "In cerevisia
Dept. of Botany - Iowa State Univ.
Ames, Iowa 50011-1020 rwallace at iastate.edu FAX:
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