Use of His10 or His 12 Tag in yeast

[Stephen Bell]

Yeasties, 

	    I was wondering whether anyone had any experience in producing His 
10 or His 12 tagged proteins in yeast?  In searching the archives I found 
one experience in which this tag appeared to be toxic.  Has anyone else 
experienced this?  On a more general note, is there any reason to believe 
that a His 10 or 12 tags work better than His 6 tags?  Thanks for any 
information. 


Steve Bell