pombe genetics question

[Jannatipour Mehrdad]

On Wed, 21 Feb 1996, Steve Ingram wrote:

> Date: Wed, 21 Feb 96 22:16:33 GMT
> From: Steve Ingram <ingram at uthscsa.edu>
> To: yeast at net.bio.net
> Subject: pombe genetics question
> 
> I am aware that gene disruptions in pombe are normally done in a diploid,
> but is there any problem with performing the disruption in a haploid, then 
> crossing the disruption strain to a WT haploid, allowing the diploid to grow
> under no selection conditions, then analyzing tetrads to be certain the
> viability is OK and that any phenotypes segregate with the disruption?
> Steve Ingram
> Dept. of Biochemistry
> UTHSCSA
> San Antonio, Tx 78284-7760
> USA
> 
What if the disruption of yfg causes lethal phenotype?
					Mehrdad Jannatipour
					University of Montreal