Direct PCR of yeast clones picked from agar plate?

Namjin Chung nc1 at acpub.duke.edu
Mon May 6 11:53:33 EST 1996


Dear yeast researchers,
I would like to know any method of direct PCR with yeast clones which are 
picked directly from agar plates.  I mean, without preparing DNA after 
growing cells in liquid culture.  

I have tried one method found in Trends in Genetics (published a few 
years ago) which is simply to PCR after transferring small portion of 
colonies.  I was not sure if it was working properly.  

I would be happy if you have any comments about this.  Thank you very 
much in advance.


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*   Namjin Chung                     |   E-mail: nc1 at acpub.duke.edu     *
*   Duke University Medical Center   |           chung006 at mc.duke.edu   *
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