Mitochondrial DNA Isolation

Bert Liang liangb at ESSEX.UCHSC.EDU
Mon Apr 7 09:00:48 EST 1997


Pat:

A restriction enzyme method with gel recovery has been published by
Wiesner et al., Gene 98:277, 1991; basically bulk DNA is cut with enzymes
which don't cut mtDNA, and the latter is recovered either in linear or
circular form.  It is pretty easy but requires alot of genomic (i.e.
nuclear + mtDNA), ~20 ug.

Hope this helps.

Bert

On 5 Apr 1997 usmbc5m3 at ibmmail.com wrote:

> 
> 
> Does anyone know of a method or kit to isolate mitochondrial DNA without
> doing a cesium gradient?
> 
> Thanks
> 
> Pat
> 
> 
> 
Bertrand C. Liang
Box B182
University of Colorado Health Sciences Center
4200 E. Ninth Ave.
Denver, CO  80262
liangb at essex.uchsc.edu




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