Yeast two-hybrid system

[Sailesh Surapureddi]

At 10.13 +0000 970213, Anne Phelan wrote:
>I have been trying to grow up HF7C yeast transformed with my bait protein in
>SD -TRP medium.  The cells are forming large aggregates following overnight
>incubation.  Has anybody seen this before?  Any ideas why this is happening?


oh yeah ! This is a common phenomenon. Just vortex it  for 60sec and
innoculate for large cultures (assuming that you are growing them on shaker
incubator at 225RPM). There should be no side effect on your culture or for
further steps. Even clonetech manual points to this aggregation phenomenon.
So there is no need to worry.

Cheers
Sailesh

Sailesh Surapureddi, Ph.D
Plan 12; Inst. for Cellbiologi
Halsouniversitetet
S 581 85, Linkoping
Sweden.

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