leu2-3,112 What are the mutations?
nc1 at acpub.duke.edu
Mon Feb 24 10:54:51 EST 1997
<Pine.OSF.3.95.970218161506.10595A-100000 at saul4.u.washington.edu>, Richard
Stevens <rstevens at u.washington.edu> wrote:
> Hey Yeasties,
> I want to integrate my gene into the LEU2 locus. The strain I'm
> working with is W303 based, and carries the leu2-3,112 allele. Does
> anyone know what the mutations are in this allele?
I heard that leu2-3,112 in W303 and other strains has two point mutations
in its ORF. FYI, ura3-52 has Ty insertion in N-terminal half. I wonder if
there are references, WWW, or whatever has list of characters of mutations
commonly found in many lab strains.
Namjin Chung, who knows little and asks a lot!
Program in Molecular Cancer Biology
Duke University Medical Center
Durham, North Carolina
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