leu2-3,112 What are the mutations?

[Namjin Chung]

In article
<Pine.OSF.3.95.970218161506.10595A-100000 at saul4.u.washington.edu>, Richard
Stevens <rstevens at u.washington.edu> wrote:

> Hey Yeasties,
>      I want to integrate my gene into the LEU2 locus.  The strain I'm
> working with is W303 based, and carries the leu2-3,112 allele.  Does
> anyone know what the mutations are in this allele?
> 
> Rick

I heard that leu2-3,112 in W303 and other strains has two point mutations
in its ORF.  FYI, ura3-52 has Ty insertion in N-terminal half. I wonder if
there are references, WWW, or whatever has list of characters of mutations
commonly found in many lab strains.


Namjin Chung, who knows little and asks a lot!
Program in Molecular Cancer Biology
Duke University Medical Center
Durham, North Carolina