2-Hybrid Vectors & Strains

[Damien hermand]

In article (Dans l'article) <339367DC.7A4C at mailbox.uq.edu.au>,
Michael.Young at mailbox.uq.edu.au wrote (écrivait) :

> Hi,
>         I am interested in using the yeast 2-hybrid system in my work.  My
> supervisor recently attended the ASM meeting in Miami where she saw a
> poster detailing some new generation vectors for this system.  They are
> pGE202 (lexA) and pJ64-5 (B42)...  Does anyone know who to contact to
> get the vectors and strains this system requires as well as more
> information required to set up a functioning 2-hybrid system???
> 
> Thanks for your assistance
> 
> Michael Young

This two-hybrid system comes from Roger Brent's lab. You wil find
informations in Gyuris et al., 1993 and in Short Protocols in Molecular
Biology (third edition)-chapter 13.