Labelling yeast in glutamine medium?

[Fergus Doherty]

I want to label yeast with 35S-met, but in a good nitrogen source. I don't
want to use ammonium sulphate (because the sulphate will be preferntially
used to make methionine - yes?).  So could I use glutamine or glutamate, if
so what concentration should I use.  What about ammonium chloride?

TIA

-- 
Fergus Doherty,
Dept Biochemistry,
Nottingham University,

Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)