integration of GFP in the yeast genome
didier Fesquet
dfesque at ns1.nimr.mrc.ac.uk
Sat Apr 18 11:46:10 EST 1998
hi, to visualize the subcellular location of a protein in the yeast
Scerevisiae, I plan to integrate a single copy of a fusion between my
gene and GFPS65T. I could not find in the litterature visualisation of
a GFP fusion expressed under its own promoter (so probably at low to
medium level!) people usually use gal driven promoter!. If anybody has
ever tried this kind of experiment , I would be happy to hear about
it! success, failure!
thanks
didier
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