his6 tag in yeast

[Justin Brown]

    before you get too far into this idea, go to the yeast genome
database and do a search for the HHHHHH amino acid sequence.  this
will show you just how many naturally His6 tagged yeast proteins will
be coming down in your column.

    justin

Antony Cooper wrote:

> Hey,
>         Can anyone send me or reference a procedure concerning the purification
> of a 6X-His tag protein from S. cerevisiae on  a Ni -column. I'd love to
> be able to lyse the cells and pour the extract  directly over the Ni-
> column.  The tagged protein is a membrane spanning protein so the
> procedure will need to tolerate a detergent of types.  Many thanks.
>
>         Antony Cooper