Yeast Spheroplasts!!

[news at nntp.stanford.edu]

We get ours from ICN, 1-800-854-0530.  Catalog #320931, US$670. for 500
mg.  We prepare stocks at 10 mg/ml in 10 mM Tris pH7.5, 1 mM EDTA, 1M
Sorbitol.  This is a suspension of Zymolyase.  Used at 15 to 100
micro-g/ml in 100 mM phosphate buffer, pH6.5, 0.2% 2-mercapto-ethanol,
with osmotic support of 1 to 1.2 M Sorbitol. It also works in Tris
buffers. 

Mike Conboy
Cyert Lab
Dept. Biological Sciences
Stanford

In article
<7o9o3u$c1d at net.bio.net>,
biosci-request at net.bio.net, Anita Udiavar Rao  <aurao at wam.umd.edu> wrote:
>
>> Hi,
>> I would like to the know the concentration of Zymase required to make
>> yeast spheroplasts.  I have the protocol from the Fission Handbook, but
>> it does not mention the make of the enzyme. It would be great if I could
>> get the right amount required.
>> 
>> Thank You
>> Anita
>> 





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca