Two hybrid / Size limitations of interacting proteins?

[Martin Wilming]

hi yeastnetters,

I´m working with a LexA two-hybrid system, which is commercially
available from invitrogen (Hybridhunter).
While studying the dimerization of a protein with approximately 800
aminoacids (that means 1600 aminoacids of the dimer) I´m wondering
about possible limitations in size of the interacting proteins. For
instance,
even interacting proteins might not give a signal with the two-hybrid system
if the steric hindrance for activation ist too high ... ? I´m wondering
because I can not find activation for the dimerization of the whole protein,
even though this dimerization is well known - but screening of some
domains of this protein results in activation ...
Does anyone know more about size limitations for the interacting proteins
to result in activation of the reporter genes ...?

thanks,
  martin

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Martin Wilming
Université de Lausanne, Bâtiment de Chimie
Institut Chimie Organique, Labo 4207
CH-1015 Lausanne-Dorigny (Suisse)

Tel.: +41-(0)21/692 40 41
Fax.: +41-(0)21/692 39 55

E-mail: martin.wilming at ico.unil.ch or kontakt at martin-wilming.de

Homepage: http://www.martin-wilming.de
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