S. pombe lysis

[Jean-Yves Masson]

Dear all:

    I would like to purify a protein from S. pombe. S. pombe cell wall is
difficult to break compared to S. cerevisiae and I would like to know what
is the best protocol to make extracts from S. pombe (large scale and
small-scale). 

    Thanks in advance for your time in responding to this message.

Jym 


________________________________________________
Jean-Yves Masson, Ph.D.
Assistant professor
Laval University Cancer Research Center
Hôtel-Dieu de Québec (CHUQ)
9, McMahon st. 
Québec (Qc)
G1R 2J6
Canada
E.mail: Jean-Yves.Masson at crhdq.ulaval.ca
Tel.: (418) 525-4444 ext 5154 (office)
Tel.: (418) 525-4444 ext 6816 (lab)
Fax: (418) 691-5439
________________________________________________



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