[Yeast] Integration in Gap1 locus / D-Histidine selection
dloque at stanford.edu
Fri Jan 20 23:46:27 EST 2006
Dear yeast users
I am trying to integrate an integrative plasmid into Gap1 locus from
Saccharomyces cerevisiae. For that propose I am using a non buffered
minimal media (0.17% YNB w/o Nitrogen, 0.1% proline, 20mg/L uracile
and 2% glucose) containing 10mM of D-Histine. However, I am getting a
huge number of colonies in my control plate (yeast transformed
Does anyone have an idea how I can decrease this background ? Could
it be a pH problem, concentration of D-Histidine ?
Many thanks in advance for your recommendations.
Carnegie Institution of Washington
Department of Plant Biology
260 Panama Street
Stanford, CA 94305
Tel. 650 325 1521 ext 249
Fax 650 325 6857
Email: dloque at stanford.edu
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Yeast