From cartera from cmp.ucsf.edu Tue Dec 2 22:42:28 2008 From: cartera from cmp.ucsf.edu (Andrew Carter) Date: Wed Dec 3 14:23:07 2008 Subject: [Yeast] Cheap yeast extract/ proteose peptone for YPD? Message-ID: Dear All I saw a thread similar to my current question back in the mid 90s, but was wondering if anyone has an update. We currently buy yeast extract and proteose peptone from Becton Dickinson (via Fisher) for making YPD. However I am beginning to do some large scale fermenter growths and the cost is getting prohibitive. I notice that there are much cheaper versions available (eg Sigma yeast extract REF 09182 is almost 5 times cheaper per kg than Bacto Yeast Extract). My question is whether anyone can recommend a good (and cheap) combination of yeast extract and proteose peptone that allows normal yeast growth (to a similar OD etc). Alternatively does anyone have any good ideas about cheap media receipes for growing yeast to very high density in a fed-batch fermenter run? Anyone ever tried using malt extract (from a brew shop - seems cheap) for a protein expression related project? Many thanks and best wishes Andrew From sandra.vergara from duke.edu Fri Dec 12 17:00:21 2008 From: sandra.vergara from duke.edu (Sandra Viviana Vergara) Date: Fri Dec 12 17:31:16 2008 Subject: [Yeast] Nucleolar Fragmentation in S. cerevisiae Message-ID: <20081212170021.cs9dxfhhpckk4cwk@webmail.duke.edu> Dear All, Does anyone know of a compound that might induce nucleolar fragmentation in S. cerevisiae? Thank you and happy holidays! Sandra Vergara -- Ph.D. candidate Genetics and Genomics Duke University From aagarwal from olemiss.edu Sat Dec 13 16:22:38 2008 From: aagarwal from olemiss.edu (Ameeta Agarwal) Date: Mon Dec 15 00:23:13 2008 Subject: [Yeast] Postdoctoral position available Message-ID: <017801c95d68$ecf17980$86a74a82@AmeetaDell> A postdoctoral research associate position is available immediately at the National Center for Natural Products Research (NCNPR) at the University of Mississippi. The project involves the use of DNA microarray technology including molecular, genetic and biochemical techniques aimed at identifying the mechanisms of action of novel natural products. We anticipate an initial duration of one year, with renewal up to three years contingent on funding and satisfactory performance. Qualifications: The applicant must have a Ph.D. in microbiology, biology, pharmacology or related discipline. Applicants should have experience in molecular biology, genetics or biochemistry. Responsibilities: The successful candidate will conduct DNA microarray experiments in yeast species, including biochemical assays and molecular/genetic studies with yeast mutants. Candidate must have excellent oral and written communication skills and should be able to work effectively in a multi-disciplinary research setting. The NCNPR is a university-affiliated research center that conducts basic and applied research to discover and develop natural products for use in pharmaceuticals, dietary supplements and agrichemicals. Salary: Commensurate with experience Applications must be submitted through the OleMiss jobsite at https://jobs.olemiss.edu For additional information, contact Dr. Ameeta Agarwal at aagarwal@olemiss.edu The University of Mississippi is an EEO/AA/Title VI/Title IX/Section 504/ADA/ADEA employer. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20081213/c9a66e93/attachment.html