[Yeast] get of ura3 mutant

[shoushou]

I am not sure whether it is diploid or haploid, but I think that it may be
diploid. Could you teach me in more detail the method using homologous
recombination of a PCR generated cassette.  


Roy Buchanan wrote:
> 
> Is it diploid or haploid? What about using homologous recombination of a
> PCR generated cassette?
> 
>  
> 
> shoushou wrote:
>> 
>> I tried to get an ura3 mutant of a yeast by EMS or MNNG mutagenesis and
>> 5-FOA selection. But I could not get it. So I would like to get it by
>> other method. Could you tell me other methods that create ura3 mutant of
>> yeast.
>> 
> 
> 

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