From S.Wang from uws.edu.au Wed Sep 3 01:07:50 2008 From: S.Wang from uws.edu.au (Shaoyu Wang) Date: Wed Sep 3 10:22:58 2008 Subject: [Yeast] Cluster gene expression data across different platforms References: <200808191704.m7JH4tU15600@net.bio.net> Message-ID: Dear All, Platforms for gene expression microarray have evolved over the time. One consequence of this "evolution" is that the number of genes across different platforms varies along with other changes. This situation makes it difficult to cluster array data obtained from different platforms. I wonder if anyone could suggest way or softwares etc to pre-process and cluster gene expression data across different platform and/or different versions of the same platform. I would appreciate any help with this. Shaun ________________________________ From: yeast-bounces@oat.bio.indiana.edu on behalf of yeast-request@oat.bio.indiana.edu Sent: Wed 20/08/2008 3:04 AM To: yeast@magpie.bio.indiana.edu Subject: Yeast Digest, Vol 39, Issue 7 Send Yeast mailing list submissions to yeast@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/yeast or, via email, send a message with subject or body 'help' to yeast-request@net.bio.net You can reach the person managing the list at yeast-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Yeast digest..." Today's Topics: 1. PEP4 deletion plasmid (Arthur Lustig) ---------------------------------------------------------------------- Message: 1 Date: Tue, 19 Aug 2008 09:14:46 -0500 From: Arthur Lustig Subject: [Yeast] PEP4 deletion plasmid To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for a pep4 deletion plasmid other than a kanr form. Liz Jones would have been the obvious source, but with her passing I am not sure of a good source. I would appreciate any help in this matter. Art Lustig Arthur J. Lustig PhD Professor, Department of Biochemistry Tulane University Health Sciences Center 1430 Tulane Avenue New Orleans, LA 70112 ph:504-988-3688 fax:504-988-3687 -------------- next part -------------- An HTML attachment was scrubbed... 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Name: not available Type: application/ms-tnef Size: 6278 bytes Desc: not available Url : http://www.bio.net/bionet/mm/yeast/attachments/20080903/6c7bb396/attachment.bin From jkellosa from mappi.helsinki.fi Mon Sep 15 15:58:35 2008 From: jkellosa from mappi.helsinki.fi (jkellosa@mappi.helsinki.fi) Date: Mon Sep 15 17:40:47 2008 Subject: [Yeast] Expression of membrane proteins in Saccharomyces Cervisae Message-ID: <20080915235835.91712y6axmu90i2j@webmail.helsinki.fi> Dear all, I have noticed that in some cases when S. cerevisae is grown in shaker flasks in order to express membrane proteins the shaking is done at quite slow speed (130-160 rpm). Has anyone any kind of clue why it is so ? Could it have something to do with inhibiting the formation of foam ? If so does anyone know if antifoaming agents are detrimental for membrane protein production. Thanks already beforehand. -Juho From maraiar from exchange.nih.gov Thu Sep 18 08:23:54 2008 From: maraiar from exchange.nih.gov (Rich Maraia) Date: Thu Sep 18 11:41:51 2008 Subject: [Yeast] Postdoc positions available Message-ID: TWO Postdoctoral Fellowship Positions available Biology of RNA Metabolism in Eukaryotes National Institutes of Health (NIH), Bethesda, Maryland The Fellows will investigate molecular mechanisms involved in RNA metabolism (this may include links between transcription, RNA processing, nuclear transport, and translation). Fission yeast serve as a model system to study human as well as yeast factors. We also use in vitro biochemistry, mammalian tissue culture and genetically altered mice. Laboratory approaches integrate cell biology, molecular biology, genetics, biochemistry and structural biology. As an applicant, you should submit a cover letter that details your specific interest in the specific research areas described above and as reflected by the publications from the Maraia lab (search PubMed for "maraia r*". Candidates must hold a Ph.D. or M.D. and have less than 5 years postdoctoral experience. Expertise in molecular biology, genetics and/or biochemistry is required, as are strong letters of personal recommendation. The successful candidate will confer regularly with the principal investigator but must incorporate self-directed research, excellent technical, presentation, and communication skills as essential parts of the job. Send your cover letter, C.V., and the names of three references with their email addresses and telephone numbers by Email to: Richard J. Maraia, M.D. Email: maraiar@mail.nih.gov 31 Center Dr., Room 2A25 Bethesda, MD 20892-2426 -- Richard J. Maraia, M.D. Captain, US Public Health Service, Commissioned Corps Chief, Section on Molecular and Cell Biology Intramural Research Program Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health 31 Center Dr., Rm 2A25 Bethesda, MD 20892-2426 Phone: 301 402-3567 Fax: 301 480-6863 http://eclipse.nichd.nih.gov/nichd/Maraia/Maraialabpage.html -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080918/a87c7668/attachment.html From kaurnipun from gmail.com Mon Sep 22 10:43:41 2008 From: kaurnipun from gmail.com (nipunjotsoni) Date: Mon Sep 22 11:51:02 2008 Subject: [Yeast] S pombe vector host system Message-ID: <9353110c0809220843n4a40e8c1tb9e73896dee29a14@mail.gmail.com> Hi everybody Myself a researcher working in India.. Can anybody please provide me with S pombe Host n its relevant expression vector system for expression of Human parathyroid hormone... Due regards nipun -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080922/48500ff3/attachment.html