From dann255 from gmail.com  Mon Aug 17 10:44:49 2009
From: dann255 from gmail.com (Dann Huh)
Date: Wed Aug 19 10:13:00 2009
Subject: [Yeast] Gentamicin in YES media (Dann Huh)
Message-ID: <5e2245e60908170844h47a906fcn11b4630bd83e771a@mail.gmail.com>

Dear All,

   I wonder if I can use Gentamicin in YES media
   for S. pombe (the fission yeast) to prevent bacterial
   contamination as we do for mammalian cells.

   Did anyone try before?

-regards,
     Dann
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From Bret.Light from bioneer.us.com  Tue Aug 18 15:07:35 2009
From: Bret.Light from bioneer.us.com (Bret Light)
Date: Wed Aug 19 10:13:05 2009
Subject: [Yeast] S. pombe glycosylation
Message-ID: <119479524866BF41A481E2EAA152A5C901ED2768@alaex01.bioneer.local>

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From akaya from genomics.unl.edu  Thu Aug 20 17:49:42 2009
From: akaya from genomics.unl.edu (Alaattin Kaya)
Date: Fri Aug 21 22:33:02 2009
Subject: [Yeast] mammalian cDNA inyeast expression vector
Message-ID: <E44987930BD14BFE849342D035AB5856@yeast6fbfd0247>

Hi

I am looking for a mammalian cDNA library cloned into a yeast
expression vector. It would be from any tissue from mouse or human.  I would greatly appreciate any help.  Thanks.

Alaattin KAYA

akaya@genomics.unl.edu

University of Nebraska-Lincoln

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From akaya from genomics.unl.edu  Mon Aug 24 13:32:19 2009
From: akaya from genomics.unl.edu (Alaattin Kaya)
Date: Wed Aug 26 08:44:45 2009
Subject: [Yeast] tet system with rtTA
Message-ID: <140B39D2803F466F884AD2AC815821B2@yeast6fbfd0247>

Hi

I am looking for a TET SYSTEM (tetracyclin induce system)    PLASMID WHICH HAS rtTA (REVERSE TRANS ACTIVATOR)  for yeast expression .  I would greatly appreciate any help.  Thanks.

Alaattin KAYA

akaya@genomics.unl.edu

University of Nebraska-Lincoln
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From philana.fernandes08 from imperial.ac.uk  Sat Aug 29 10:02:19 2009
From: philana.fernandes08 from imperial.ac.uk (Fernandes, Philana)
Date: Sat Aug 29 23:13:00 2009
Subject: [Yeast] No background with yeast two-hybrid screen
Message-ID: <04B01B0BED4DD34DAAB527E528DD42F20FB56DFA43@ICEXM1.ic.ac.uk>

Hi Jennifer,

I have just recently performed a Y2HG screen and found that the plating volume of 200microlitres resulted in plates that were too dense - so much so, it looked like a lawn and individual colonies could not been seen at all.

I simply patched areas from this plate onto fresh plates and voila - single colonies.

Hope this helps....
Philana
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