From mukeshpyadava from gmail.com Sat May 2 00:58:07 2009 From: mukeshpyadava from gmail.com (Mukesh Yadav) Date: Sun May 3 09:20:11 2009 Subject: [Yeast] query for highly transformable S.Pombe cell Message-ID: <36419f5a0905012258x19df907ey78d52da8bd3b4e62@mail.gmail.com> I am extremly sorry for my blunder in my previous mail regarding highly transformable S.Pombe vector. By mistake it was written 'Vector' instead of ' Cells '. I apologise for that. That should be read *"highly transformable S.Pombe cell"* as that VL6-48N of S. cerevisiae. (ref. Natalay Kouprina, vladimir N. Noskov and Vladimir Larionov :methods in Molecular Biology, Vol 349). -- Mukesh Pratap Yadava C/O Prof. D.D. Dubey Molecular Biology Laboratory Department of Biotechnolgy Faculty Building VBS Purvanchal University Jaunpur-222001 (Uttar pradesh) Mob. No- +91-9451157085 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20090501/ceb821a0/attachment.html From rahulmishra72 from gmail.com Wed May 6 02:07:01 2009 From: rahulmishra72 from gmail.com (rahul mishra) Date: Sat May 9 08:24:35 2009 Subject: [Yeast] query for mutant cells Message-ID: Does anybody have "D18 cds1? ura- s.pombe cells" and "D18 rad3? ura- s. pombe cells" Kindly tell how to get -- - Rahul Kumar Mishra c/o Prof. D.D. Dubey Molecular Biology Laboratory, Dptt. of Biotechnology, VBS Purvanchal University, Jaunpur-222001 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20090506/659c5a52/attachment.html From tsdavis1 from gmail.com Wed May 20 20:15:40 2009 From: tsdavis1 from gmail.com (Thomas Davis) Date: Thu May 21 08:42:40 2009 Subject: [Yeast] determining growth rate Message-ID: <1549e6fe0905201815l16c3c23ahbce4c83f879c6ff3@mail.gmail.com> Hi everyone, I am an entomologist trying to study the microbial community associated with an herbivorous beetle species. As such, our lab is rather limited in terms of microbiological sophistication. We have tentatively isolated a wealth of yeast colonies from the glandular mycangia of these insects, and would like to conduct some performance-type experiments using our yeast isolates and chemical treatments. Our isolations are currently being done on 2% MEA, and appear to mostly be in the genera Pichia, Saccharomyces, and Candida. My question to the group is this: What is a reliable, quick, and easily interpreted method for assessing growth rates of yeast colonies? We are familiar with the technique of colony forming unit count via serial dilutions, however, this seems to be a laborious means of assessing growth rate over time with many strains and (potentially) multiple chemical treatments. Or is it simply best to try to obtain a single 'snapshot' of growth rate? Any advice that could be offered would be greatly appreciated. Thank you. Seth Davis -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20090520/fe04a475/attachment.html From jnjasmin from gmail.com Fri May 22 09:59:58 2009 From: jnjasmin from gmail.com (Jean-Nicolas Jasmin) Date: Fri May 22 18:34:55 2009 Subject: [Yeast] spores isolation Message-ID: <5fb1ce6b0905220759t27cdac2ei9d5480154669a463@mail.gmail.com> Hi all, I would like to isolate spores of S. cerevisiae from a mixed culture containing spores and vegetative (haploid and some diploid) cells. I obtain that culture by digestion with zymolyase (which, by the way, is rather inefficient at killing vegetative cells of my strain). I am currently using mineral oil and water: spores segregate in the oil and cells segregate in the water. But it is almost impossible to get the spores out of the oil. Even centrifuging at 13,000 rpm for over 20 mins won't transfer them from the oil into water. Ultimately, my goal is to transfer the spores (with almost no vegetative cells) into liquid growth medium. Any suggestions on how I can isolate the spores? I have checked the archive from the yeast list and I couldn't find a solution to that problem. Thank you in advance, Jean-Nicolas -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20090522/20d1aa04/attachment.html From tsdavis1 from gmail.com Fri May 22 17:08:33 2009 From: tsdavis1 from gmail.com (Thomas Davis) Date: Fri May 22 18:35:01 2009 Subject: [Yeast] Liquid media? Message-ID: <1549e6fe0905221508y5abe03b2j294d733469a17287@mail.gmail.com> Hi again everyone, and thanks for all the previous responses to my growth rate question. We have already tried to assess yeast growth rates using OD600 in TSB (tryptic soy broth) media. We got rather spurious results, with the OD jumping all over the place between measurements. What is the appropriate liquid media to use for Pichia, Candida, and Saccharomyces? We are worried that our previous dubious results may be due to using the wrong kind of liquid media. Once again, any advice on the appropriate media/technique would be greatly appreciated. Thanks again, Seth Davis -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20090522/f1354bfd/attachment.html