From mukeshpyadava from gmail.com  Mon Nov  9 06:42:29 2009
From: mukeshpyadava from gmail.com (Mukesh Yadav)
Date: Mon Nov  9 09:08:58 2009
Subject: [Yeast] Query Regarding Plasmid isolation protocol
Message-ID: <36419f5a0911090342t865b48bma1140dab44613eaf@mail.gmail.com>

Does anybody preferably know the plasmid isolation from fission yeast?

Mukesh Pratap Yadav
Graduate student
C/O Prof. D.D. Dubey
Molecular Biology Laboratory
Department of Biotechnolgy
VBS Purvanchal University
Jaunpur-222001 (Uttar pradesh)
India.
Mob. No- +91-9451157085
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From multanova from gmail.com  Fri Nov 20 13:20:21 2009
From: multanova from gmail.com (Meg)
Date: Fri Nov 20 14:18:57 2009
Subject: [Yeast] Slightly off topic: are home-baking and work-cell-culture 
	incompatible?
Message-ID: <d9fa828e0911201020s57887ed1m8c6209879e6ed108@mail.gmail.com>

Hi everyone,

I've got a bit of a quandary and I'd like to hear your input.  My
roommate bakes bread, and I do cell culture at the lab.  I know that
some labs have experienced yeast contamination of their cell lines,
attributed to baking or brewing at home.

Do you have any particular tips, other than stringent good lab
practice, that can really help prevent any contamination?  I can't
prohibit her from baking (nor do I want to), but are there any special
precautions that she/we can take at home?

And from your experience: are there any of you who do bake bread at
home, without problems at work?  (am I worrying overly much?)

Thanks in advance,
Megan Barker

From maldegem from mrc-lmb.cam.ac.uk  Wed Nov 25 13:53:00 2009
From: maldegem from mrc-lmb.cam.ac.uk (Febe van Maldegem)
Date: Thu Nov 26 10:27:01 2009
Subject: [Yeast] Phloxine B staining: diploidy or mutant phenotype?
Message-ID: <4B0D7D0C.8050603@mrc-lmb.cam.ac.uk>

Dear colleagues,

I have a temperature sensitive mutant strain, that also grows in red 
colonies on Phloxine B plates at 30C.
How do I distinguish diploidy from a reduced viability due to the 
mutation? In other words, how can I reassure myself that I have a 
haploid mutant strain?

Thanks in advance!
Febe van Maldegem
MRC-LMB, Cambridge, UK

From akaya from genomics.unl.edu  Thu Nov 26 13:55:18 2009
From: akaya from genomics.unl.edu (Alaattin Kaya)
Date: Fri Nov 27 10:43:53 2009
Subject: [Yeast] Re: Yeast Digest, Vol 54, Issue 3
References: <200911261706.nAQH6SM27156@net.bio.net>
Message-ID: <4C7FB4E9460947599D62C2F98D17A8CA@yeast6fbfd0247>

Hi ;
Use sporulation media for your mutant strain. this is the easy way I think. 
on sporulation media if there is a spores that means your strain is somehow 
diploid. You can check under microscope.

Alaattin KAYA


----- Original Message ----- 
From: <yeast-request@oat.bio.indiana.edu>
To: <yeast@magpie.bio.indiana.edu>
Sent: Thursday, November 26, 2009 11:06 AM
Subject: Yeast Digest, Vol 54, Issue 3


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>   1. Phloxine B staining: diploidy or mutant phenotype?
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> Message: 1
> Date: Wed, 25 Nov 2009 18:53:00 +0000
> From: Febe van Maldegem <maldegem@mrc-lmb.cam.ac.uk>
> Subject: [Yeast] Phloxine B staining: diploidy or mutant phenotype?
> To: yeast@magpie.bio.indiana.edu
> Message-ID: <4B0D7D0C.8050603@mrc-lmb.cam.ac.uk>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Dear colleagues,
>
> I have a temperature sensitive mutant strain, that also grows in red
> colonies on Phloxine B plates at 30C.
> How do I distinguish diploidy from a reduced viability due to the
> mutation? In other words, how can I reassure myself that I have a
> haploid mutant strain?
>
> Thanks in advance!
> Febe van Maldegem
> MRC-LMB, Cambridge, UK
>
>
>
> ------------------------------
>
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